Scoresby S, Ramos S, Walker F, Go R, Quinn C.  2024. Understanding the Full-Taxa Diet of Sierra Nevada Red Foxes (Vulpes vulpes necator) through Fecal DNA Metabarcoding.  Poster for the 31st Annual Wildlife Society Conference. October 22, 2024, Baltimore, MD.

Collaborators

A million thanks to my collaborators at Cascadia Wild, Oregon Department of Fish and Wildlife, Cory Farstveldt at Deschutes National Forest, Dr. Katie Moriarty of the National Council on Air and Stream Improvement, and big special thanks to Marwa Mahmoud of Washington State University’s Mammal Spatial Ecology Lab for sharing scats with me.

Thank you Jesse, Osqar, Noni, and Edward for being my incredible field crew.

Thanks also to my funders at the Alfred P. Sloan Foundation, U.S.D.A. National Needs Foundation, Northern Arizona University, and the Oregon Wildlife Foundation.

Poster flowchart image partially created in BioRender, www.biorender.com

Fox dinner party (methods image 7) created with AI assistance from ChatGPT, OpenAI

References

Ando, H., Mukai, H., Komura, T., Dewi, T., Ando, M., Isagi, Y., 2020. Methodological trends and perspectives of animal dietary studies by   noninvasive fecal DNA metabarcoding. Environ. DNA 2, 391–406.

Aubry, K.B., Statham, M.J., Sacks, B.N., Perrine, J.D., Wisely, S.M., 2009. Phylogeography of the North American red fox: vicariance in Pleistocene   forest refugia. Mol. Ecol. 18, 2668-2668.

da Silva, L.P., Mata, V.A., Lopes, P.B., Pereira, P., Jarman, S.N., Lopes, R.J., Beja, P., 2019. Advancing the integration of multi-marker   metabarcoding data in dietary analysis of trophic generalists. Mol. Ecol. Resour. 19, 1420–1432.

Drake, L.E., Cuff, J.P., Young, R.E., Marchbank, A., Chadwick, E.A., Symondson, W.O., 2022. An assessment of minimum sequence copy thresholds   for identifying and reducing the prevalence of artefacts in dietary metabarcoding data. Methods Ecol. Evol. 13, 694–710.

Ernakovich, J.G., Hopping, K.A., Berdanier, A.B., Simpson, R.T., Kachergis, E.J., Steltzer, H., Wallenstein, M.D., 2014. Predicted responses of arctic   and alpine ecosystems to altered seasonality under climate change. Glob. Change Biol. 20, 3256-3269.

Galan, M., M. Pagès, and J.-F. Cosson. 2012. Next-generation sequencing for rodent barcoding: species identification from fresh, degraded and environmental samples. PLoS One 7:e48374.

Goldberg, A.R., Conway, C.J., Tank, D.C., Andrews, K.R., Gour, D.S., Waits, L.P., 2020. Diet of a rare herbivore based on DNA   metabarcoding of   feces: Selection, seasonality, and survival. Ecol. Evol. 10, 7627-7643.

Green, G.A., Sacks, B.N., Erickson, L.J. and Aubry, K.B., 2017. Genetic characteristics of red foxes in northeastern Oregon. Northwestern Naturalist,   98(2), pp.73-81.

Griffith, D. M., J. A. Veech, and C. J. Marsh. 2016. Cooccur: probabilistic species co-occurrence analysis in R. Journal of Statistical Software 69:1–17.

Hisano, M., Evans, M.J., Soga, M., Tsunoda, H., 2022. Red foxes in Japan show adaptability in prey resource according to geography and season: A   meta-analysis. Ecol. Res. 37, 197–214.

Newsome, T.M., Ripple, W.J., 2015. A continental scale trophic cascade from wolves through coyotes to foxes. J. Anim. Ecol. 84, 49–59.

Perrine, J.D., n.d. Ecology of red fox (Vulpes vulpes) in the Lassen Peak region of California, United States of America (Ph.D.). University of   California, Berkeley, United States -- California.

Perrine, J.D., Pollinger, J.P., Sacks, B.N., Barrett, R.H., Wayne, R.K., 2007. Genetic evidence for the persistence of the critically endangered Sierra   Nevada red fox in California. Conserv. Genet. 8, 1083–1095.

Pompanon, F., B. E. Deagle, W. O. C. Symondson, D. S. Brown, S. N. Jarman, and P. Taberlet. 2012. Who is eating what: diet assessment using next generation sequencing. Molecular Ecology 21:1931–1950.

Quinn, C.B., Akins, J.R., Hiller, T.L., Sacks, B.N., 2018. Predicting the Potential Distribution of the Sierra Nevada Red Fox in the Oregon Cascades. J.   Fish Wildl. Manag. 9, 351–366.

Quinn, C.B., Alden, P.B., Sacks, B.N., 2019. Noninvasive sampling reveals short-term genetic rescue in an insular red fox population. J. Hered. 110,   559–576.

Quinn, C.B., Preckler-Quisquater, S., Akins, J.R., Cross, P.R., Alden, P.B., Vanderzwan, S.L., Stephenson, J.A., Figura, P.J., Green, G.A., Hiller, T.L.,   Sacks, B.N., 2022. Contrasting genetic trajectories of endangered and expanding red fox populations in the western U.S. Heredity 129,   123–136.

Ripple, W.J., Wirsing, A.J., Wilmers, C.C., Letnic, M., 2013. Widespread mesopredator effects after wolf extirpation. Biol. Conserv. 160, 70–79.  

Roemer, G.W., Gompper, M.E., Van Valkenburgh, B., 2009. The ecological role of the mammalian mesocarnivore. BioScience 59, 165–173.

Taberlet, P., Coissac, E., Pompanon, F., Brochmann, C., Willerslev, E., 2012. Towards next-generation biodiversity assessment using DNA   metabarcoding. Mol. Ecol. 21, 2045–2050.

Tercel, M.P.T.G., Symondson, W.O.C., Cuff, J.P., 2021. The problem of omnivory: A synthesis on omnivory and DNA metabarcoding. Mol. Ecol. 30, 2199-2206.

Tosa, M.I., Lesmeister, D.B., Allen, J.M., Levi, T., 2023. Multi‐locus DNA metabarcoding reveals seasonality of foraging ecology of western spotted   skunks in the Pacific Northwest. Ecosphere 14, e4386.

Wierzbowska, I., Skalski, T., 2010. Fox and martens–are they really opportunistic feeders? A case of beetles and other arthropods occurrence in   carnivores’ diet. Balt. J. Coleopterol. 10, 129–139.